How does adipose derived Mesenchymal stem cells differ from bone marrow?

A comparison of biological characteristics and multilineage differentiation of bone marrow and adipose-derived Mesenchymal stem cells.

We compared the two sources of adipose and bone marrow-derived mesenchymal stem cells (BMSCs and AMSCs ) in multiple differentiation capacity and biological characteristics to provide a theoretical basis for stem cells transplantation.

We isolated bone marrow- and adipose-derived mesenchymal stem cells and compared their phenotype,cell doubling time, the secretion of factors and their ability of multi-differentiation. We also compared their differences in T-lymphocyte activation, proliferation, and suppression. BMSCs and AMSCs were similar in cell phenotype and the differences existed only in the expression of CD106.

On the proliferation rate, AMSCs were faster than BMSCs (doubling time 28 vs. 39 h). In addition, both of these two sources of cells were able to differentiate into bone, fat and cartilage that proved their stem cells properties and the number of stem cell progenitors (CFU-F) from adipose tissue were 10 times larger than those from bone marrow. But AMSCs showed a diminished capacity for suppressing T lymphocyte proliferation and activation compared to BMSCs. Cell origin and abundance were decisive factors in stem cells applications and, in the same volume, with the same premise of AMSCs and BMSCs, adipose tissue is a more promising source of stem cells.

Source: Department of Internal Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing, 100038, China.

The comparison of multilineage differentiation of bone marrow and adipose-derived mesenchymal stem cells.

BACKGROUND:
To compare the roles of adipose and bone marrow derived mesenchymal stem cells (AMSCs and BMSCs) in multiple differentiation capacity to provide a theoretical basis for stem cell transplantation.

METHODS:
We isolated bone marrow and adipose-derived mesenchymal stem cells and compared their phenotype, cell doubling time, the secretion of factors, and the ability of multi-differentiation.

RESULTS:
BMSCs and AMSCs were similar in cell phenotype and the differences existed only between the expression of CD106. The proliferation rate of AMSCs was faster than of BMSCs (doubling time 28h vs. 39h) and the capacity to suppress T cell proliferation and activation was weakened in AMSCs. In addition, both sources of cells were able to differentiate into bone, fat, and cartilage which proved their stem cell properties.

CONCLUSIONS:
Cell origin and abundance were decisive factors in stem cell applications and with the same premise as for AMSCs and BMSCs, adipose tissue is a more promising source of stem cells.

Source: Clinical Research Center, Affiliated Hospital of Guangdong Medical College, China.